Complete Chloroplast Genome Sequence of Fortunella venosa (Champ. ex Benth.) C.C.Huang (Rutaceae): Comparative Analysis, Phylogenetic Relationships, and Robust Support for Its Status as an Independent Species

Fortunella venosa (Rutaceae) is an endangered species endemic to China and its taxonomic status has been controversial. The genus contains a variety of important economic plants with high value in food, medicine, ornamental. However, the placement Genus into Citrus led controversy on taxonomy Systematics. In this present research, Chloroplast genome F. was sequenced using second-generation sequencing, structure phylogenetic relationship analyzed. results showed that size 160,265 bp, typical angiosperm four-part ring containing large single copy region (LSC) (87,597 bp), small (SSC) (18,732 pair inverted repeat regions (IRa\IRb) (26,968 bp each). There are 134 predicted genes genome, including 89 protein-coding genes, 8 rRNAs, 37 tRNAs. GC-content whole 43%, IR having higher GC content than LSC SSC regions. were no rearrangements genome; however, obvious contraction expansion. A total 108 simple sequence repeats (SSRs) entire chloroplast nucleotide polymorphism SSC. addition, there preference for codon usage non-coding being more conserved coding Phylogenetic analysis nested independent supported robustly, which significantly different from japonica. These findings will help development DNA barcodes can be useful study systematics evolution family Rutaceae.